Stable Cell Line Construction

The establishment of stable cell lines with high and reproducible protein expression is a crucial step in protein/antibody production, both for process optimization and for sustained productivity. The process is both time consuming and empirical where accumulated experiences often are needed to achieve high success rate.

ProteinCT Biotechnologies provides reliable stable cell line services, backed by years of experience, our unique codon optimization and verification capabilities, proprietary optimized vectors and validated processes, to meet your protein/antibody production needs.


Key Features:

  • Optimized expression vector for stable and high expression cell line construction
  • Reliable Codon Optimization and verification for enhanced expression
  • Thorough assessment of cell line stability for sustainable production
  • Cell-identification and banking services for reproducible results


Service List:

Milestones Specifications Deliverables Turnaround Time
Vector construction
  • • Codon optimized gene synthesis
  • • Subcloning into desired vector
  • • Transfection grade plasmid prep
Vector Sequence 5 weeks
Expression evaluation Transient transfection followed by protein expression analysis Expression report 3 weeks
Stable cell line generation
  • • Selection of stable clones, subcloning
  • • Expansion
  • • Expression test by Western blotting
  • • Selection report
  • • Top 3 expression stable cell lines
20 weeks
Stability evaluation(Optional) Evaluate stable cell line growth rate, proliferation and protein expression Evaluation report Project dependent
Cell banking(Optional) Preparation and characterization of master and working cell banks Master and working cell banks Project dependent


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Quotation & Ordering:

  • For quotation requests and questions, you may contact us by email, phone, or via our secured online message system.
  • To order, simply download the quotation/order form and submit to: Orders can be placed by phone, email, fax, or online with a formal PO (Purchase Order) . You may contact us anytime for assistance.


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