Modification & Conformation Analysis

This service portfolio covers protein posttranslational modification analysis (eg, phosphorylation, glycosylation and methylation), protein conformation analysis by Circular Dichorism (CD) and the analysis of metal co-factors.

Protein posttranslational modification (PTM) analysis

Customer provide Service specifications Deliverables Turnaround time Price
  • • 5-10µg protein on a gel spot, or 50µg or more pure protein in solution
  • • Description of protein
Tryptic digestion followed by LC/MS/MS for peptide mixture Detailed report of the analysis Within 3 weeks after sample is received Quote

 

Protein conformation analysis service

Circular dichroism (CD) spectroscopy is a standard tool for protein/peptide conformational analysis. For proteins and peptides, the spectra in the far UV region (<260 nm) is usually dominated by the absorbance 1--of the peptide bonds, correlating to protein secondary structure content. In addition, CD bands above 260 nm originate from the aromatic residues and disulfide bonds, and interactions within their immediate environments, indicating correct folding of a protein target. CD is also an ideal method for protein mutational analysis.

Customer provide Service specifications Deliverables Turnaround time Price
  • • Purified protein sample, at least 1ml of 0.5 mg/ml or higher concentration
  • • Control buffer sample
  • • Conditions to be tested
  • • CD analysis of sample under specified conditions
  • • Custom experimental design
Report of protein conformation analysis Within 3 weeks after sample is received Quote

 

Protein metal co-factor analysis service

Protein often depends on cofactors for function, where metals are a class of key protein cofactors. Also, proteins purified through IMAC method often contain unwanted metals that may interfere with their function and down-stream applications. Our elemental analysis service will provide a metal-profile for your protein sample, and our experienced service specialist is ready to provide you professional assistance in analyzing the data.

Customer provide Service specifications Deliverables Turnaround time Price
  • • Purified protein sample, 100µg or more
  • • Control buffer sample
  • • Metal(s) of interest
Elemental analysis of the samples by ICP-OES Full report of the concentration of each tested elements Within 3 weeks after sample is received Quote

* Metals covered under this service: Zinc, Manganese, Copper, Iron, Sodium, Aluminum, Cadmium, Chromium, Cobalt, Lithium, Molybdenum, Nickel, Lead, Titanium, Vanadium and Selenium.

 

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Quotation & Ordering:

  • For quotation requests and questions, you may contact us by email, phone, or via our secured online message system.
  • To order, simply fill out the order/quotation form and submit to: order@protein-ct.com. Orders can be placed by phone, email, fax, or online with a formal PO (Purchase Order) . You may contact us anytime for assistance.

 

 
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