CrystalClear Structure Service

We offer a milestone based structure determination service by X-ray crystallography. The progress of every project is evaluated at each milestone point and communicated to the customer in a timely fashion. Delivery timeline is project dependent.

Send us your target of interest and Quote today to have the structure solved.

Phase of Project Milestones Service Specifications Deliverables
I. Protein production Cloning and expression optimization
  • • Gene synthesis with codon optimization
  • • Subclone into expression vector(s)
  • • Expression screening
  • • Expression condition optimization
  • • Report of fully optimized sequence
  • • Record of cloning and expression screening optimization
Scale-up protein production
  • • Protein expression at scales for generating sufficient quantities of target protein
  • • Optimized purification for high quality protein production
  • • QC by SDS-PAGE
High purity protein >95% pure, ready for crystallization screening
II. Crystallization Crystallization Screening
  • • Crystal Screening HTP screening under 1536 conditions and/or
  • • Manual screening using standard kit analysis
Report of results under crystallization conditions
Crystallization optimization Manual condition optimization to generate diffraction crystals Protein Crystal
III. Crystal structure determination X-ray data collection Collection of high-resolution X-ray data X-ray data
Crystal structure determination Structure determination proprietary automated crystallography software and manual optimization
  • Density map
  • Protein structure ready for PDB deposition
  • Structure statistics
IV. Protein complex analysis(optional) Co-crystallization
  • • Soaking crystal with compound(s)
  • • Co-crystallization of protein complex
Protein complex crystal
X-ray data collection Collection of high-resolution X-ray data X-ray data
Crystal structure determination Structure determination with crystallography softwares
  • • Density map
  • • Protein structure (PDB format)
  • • Structure statistics

 

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Quotation & Ordering:

  • For quotation requests and questions, you may contact us by email, phone, or via our secured online message system.
  • To order, simply fill out the order/quotation form and submit to: order@protein-ct.com. Orders can be placed by phone, email, fax, or online with a formal PO (Purchase Order) . You may contact us anytime for assistance.

 

 
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