SAR-By-NMR Service
Nuclear Magnetic Resonance (NMR) is a technique for solving protein and nucleic acid structures as well as evaluating protein dynamics and complex formation. We offer a milestone based structure determination service by NMR spectroscopy, Structure-Activity-Relationships-by-NMR, with options for protein compound binding screenings. The progress of each project is evaluated at each milestone point and communicated to the customer. Delivery timeline is project dependent.
Send us your target of interest and Quote today to have the structure analyzed.
Phase of Project | Milestones | Service Specifications | Deliverables |
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I. Protein production | Cloning and expression optimization |
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15N/13C Isotope labeling and protein production |
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High purity 15N/13C Isotope labeled protein >95% pure, ready for NMR experiments | |
II. NMR structure determination | Folding screening by 15N HSQC | 15N HSQC screening for best data collection conditions | Report of screening conditions and results, including 15N HSQC spectra |
NMR data collection |
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NMR data | |
NMR solution structure calculation | Structure determination with proprietary automated and manually adjusted software programs | Structure ensemble | |
III. Protein dynamics, disorder, stability analysis (optional) | Data collection | Data collection | NMR data |
Analysis of user-specified parameter | Determination of requested parameters | Detailed report of user-specified parameters | |
IV. Protein Complex determination and functional analysis; Protein-Protein , Protein/Nucleic Acid complex analysis (optional) | Preparation of protein complex |
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NMR data and report |
Protein binding site determination |
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Report of binding characteristics including structure representation | |
Complex structure determination | Milestones and steps refer to phase I-III |
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Related Products:
- Codon Optimization & Verification • mAb Library Service
- Gene-to-Protein Service • Protein Blotting/Staining System
Quotation & Ordering:
- For quotation requests and questions, you may contact us by email, phone, or via our secured online message system.
- To order, simply fill out the order/quotation form and submit to: order@protein-ct.com. Orders can be placed by phone, email, fax, or online with a formal PO (Purchase Order) . You may contact us anytime for assistance.